I'm no expert with wine, but it looks like you might have a release mismatch in lines 64 and 65. Line 65 is commented out so it shouldn't be affecting anything, but line 64 might be a problem.
64 deb Index of /wine-builds/ubuntu/dists/hirsute/main focal main
65 # deb-src Index of /wine-builds/ubuntu/dists/hirsute/main focal main
Notice that hirsute is release 21.04 and focal is 20.04.
To test it out you can try commenting out line 64 and see if that eliminates the error message.
To do that using a graphical text editor:
- Open the Terminal app from the menu. (Or press Ctrl+Alt+t on your keyboard.)
- Type the following command:
sudo pluma /etc/apt/sources.list
("Sudo" tells the Pluma app to open the sources.list file as an administrator.)
- Enter your password when prompted. Pluma then opens the file.
- Add a "#" character at the beginning of line 64. It should now read:
64 # deb Index of /wine-builds/ubuntu/dists/hirsute/main focal main
- Save the file.
If that fixes the issue, you could remove the "#" and try changing the word "focal" to "hirsute" in line 64. As I mentioned, I'm no expert with Wine, but I don't think there should be a mismatch.